Buchi Encapsulator B-395 Pro User Manual

11593484 enEncapsulatorOperation ManualB-395 Pro

2 Safety10 B-395 Pro Operation Manual, Version C NoticeRisk of instrument damage by wrong mains supply.• External mains supply must meet the voltage

2 Safety11 B-395 Pro Operation Manual, Version C2.6 General safety rulesResponsibility of the operatorThe head of laboratory is responsible for train

3 Technical data12 B-395 Pro Operation Manual, Version C3 Technical dataThis chapter introduces the reader to the instrument and its specifications.

3 Technical data13 B-395 Pro Operation Manual, Version C3.1.2 Standard accessoriesTable 3-2: Standard accessoriesProduct Order no.Reaction vessel 11

3 Technical data14 B-395 Pro Operation Manual, Version C3.1.3 Optional accessoriesTable 3-3: Optional accessoriesProduct Order no.Concentric nozzle

3 Technical data15 B-395 Pro Operation Manual, Version C3.2 Technical dataTable 3-5: Technical data Encapsulator B-395 ProPower consumption max. 150

4 Description of function16 B-395 Pro Operation Manual, Version C4 Description of functionThis chapter explains the basic working principle of the E

4 Description of function17 B-395 Pro Operation Manual, Version C 576821161718 19121113141510943Figure 4-1: Schematic representation of the Encapsula

4 Description of function18 B-395 Pro Operation Manual, Version CThe main parts of the Encapsulator B-395 Pro are the control unit, with the syringe

5 Putting into operation19 B-395 Pro Operation Manual, Version C5 Putting into operationThis chapter describes how the instrument has to be installe

5 Putting into operation20 B-395 Pro Operation Manual, Version C5.2 Installing the Encapsulator B-395 ProPlace the instrument on the lab bench with

5 Putting into operation21 B-395 Pro Operation Manual, Version CInstallation of the air lineA 3 m air tube (2.6×4.0 mm) is included with each Encapsu

5 Putting into operation22 B-395 Pro Operation Manual, Version C5.4 Assembling of the reaction vesselThe Reaction vessel forms a closed, autoclavabl

5 Putting into operation23 B-395 Pro Operation Manual, Version C5.4.1 Cover plateThe cover plate is delivered with all pieces in place. Before use,

5 Putting into operation24 B-395 Pro Operation Manual, Version C5.4.1.1 Bead producing unit and nozzles5 64139827a Magnet Holder * O-Ring (14×1.78)

5 Putting into operation25 B-395 Pro Operation Manual, Version C5.4.1.2 Electrode324561a Screw nut M10 (polyamide) O-Ring (10.82×1.78) Insulator E

5 Putting into operation26 B-395 Pro Operation Manual, Version C5.4.1.4 Air filter1432a HEPA Air filter O-Ring 3.68×1.78 Nipple Silicon tube 5×8

5 Putting into operation27 B-395 Pro Operation Manual, Version C5.4.2 Base plateThe base plate serves a dual function with two evacuation ports. One

5 Putting into operation28 B-395 Pro Operation Manual, Version C5.4.2.1 Bead drain valve1256743a Plunger O-Ring (5×1) Knob for valve Screw M3×6

5 Putting into operation29 B-395 Pro Operation Manual, Version C5.4.3 Bead collecting flaskAfter finishing bead production and bead processing, the be

Table of contents3 B-395 Pro Operation Manual, Version CRead this manual carefully before installing and running your system and note the safety preca

5 Putting into operation30 B-395 Pro Operation Manual, Version C5.5 Pumping systemsThe Encapsulator B-395 Pro provides two systems for pumping the i

5 Putting into operation31 B-395 Pro Operation Manual, Version C5.5.2 Pressure bottleThe pressure bottle is an autoclavable container used to pump t

5 Putting into operation32 B-395 Pro Operation Manual, Version C5.5.3 Installation of the pressure bottle1. Assemble and – if needed - autoclave th

5 Putting into operation33 B-395 Pro Operation Manual, Version C5.6 Option: Concentric nozzle systemThe concentric nozzle system (CN system) is an o

5 Putting into operation34 B-395 Pro Operation Manual, Version CFigure 5-21: CN bead producing unit with set of 7 shell nozzles. The following nozzle

5 Putting into operation35 B-395 Pro Operation Manual, Version C5.6.1 Mounting of CN nozzles12Put the O-ring 12.42×1.78 in the grove of the CN bead

5 Putting into operation36 B-395 Pro Operation Manual, Version C5.7 All parts of the Encapsulator B-395 ProFigure 5-26: Picture of all parts of the

6 Operation37 B-395 Pro Operation Manual, Version C6 OperationThis chapter gives examples of typical instrument applications and instructions on how

6 Operation38 B-395 Pro Operation Manual, Version C6.3 Menu structure of the control unitThe figure below shows a schematic overview of all menus of t

6 Operation39 B-395 Pro Operation Manual, Version C6.4 Menu functions of the upper touch screenVibration (frequency and amplitude), electrostatic dis

Table of contents4 B-395 Pro Operation Manual, Version C5.5.3 Installation of the pressure bottle . . . . . . . . . . . . . . . . . . . . . . . . .

6 Operation40 B-395 Pro Operation Manual, Version CThe light intensity of the stroboscope lamp and amplitude ( = intensity) of the vibration can be se

6 Operation41 B-395 Pro Operation Manual, Version C6.5 Menu functions of the lower touch screenSyringe pump (pump speed and calibration) and magnetic

6 Operation42 B-395 Pro Operation Manual, Version CPressing on the (+) and (–) buttons will change the pump-ing rate. Pressing “Esc” will return you t

6 Operation43 B-395 Pro Operation Manual, Version CPressing on the (+) and (–) buttons for entering the liquid pumped for 1 min. Then press button “st

6 Operation44 B-395 Pro Operation Manual, Version C6.6 Manual air pressure controlIn the control unit the pressure is manually controlled by the pres

6 Operation45 B-395 Pro Operation Manual, Version C6.7 Handling the syringe pumpWhen the syringe pump is used the first time after switching on the co

6 Operation46 B-395 Pro Operation Manual, Version C6.8 Practicing with the Encapsulator, using waterBefore working with encapsulation polymers, use w

6 Operation47 B-395 Pro Operation Manual, Version CTable 6-1: Determination of the working field (with syringe pump)Nozzle size: Syringe size:Pumping S

6 Operation48 B-395 Pro Operation Manual, Version C4. Set the pumping speed and the vibration frequency to the values you determined that creates a g

6 Operation49 B-395 Pro Operation Manual, Version C6.8.2 Using the pressure bottle1. Assemble the bead producing unit, screw the 0.30 mm single nozz

1 About this manual5 B-395 Pro Operation Manual, Version C1 About this manualThis manual describes the Encapsulator B-395 Pro. It provides all infor

6 Operation50 B-395 Pro Operation Manual, Version CTable 6-2: Determination of the working field (with pressure bottle)Nozzle size:Air pressure Clear b

6 Operation51 B-395 Pro Operation Manual, Version C7. Set the liquid flow rate and the vibration frequency to a value where a clear bead chain is obta

6 Operation52 B-395 Pro Operation Manual, Version C6.9 Practicing with the Encapsulator, using non-sterile alginate solutionAfter getting comfortable

6 Operation53 B-395 Pro Operation Manual, Version C6.9.2 Working with the syringe pump1. Attach a 200 µm or 300 µm nozzle to the bead producing unit

6 Operation54 B-395 Pro Operation Manual, Version CTable 6-4: Encapsulator trial test work sheet (syringe pump)Syringe size [mL]Nozzle size [µm]Algina

6 Operation55 B-395 Pro Operation Manual, Version CTable 6-5: Determination of the working fieldNozzle size: Alginate concentration: Syringe size:Pumpi

6 Operation56 B-395 Pro Operation Manual, Version C6.9.3 Working with the pressure bottle1. Attach a 200 µm or 300 µm nozzle to the bead producing u

6 Operation57 B-395 Pro Operation Manual, Version CNOTEThe stronger the circular dispersal of the bead stream the better is the bead homogeneity. This

6 Operation58 B-395 Pro Operation Manual, Version C6.10 Practicing with the Encapsulator, working with the complete reaction vesselAfter getting comf

6 Operation59 B-395 Pro Operation Manual, Version C6. As soon as the bead chain is stable, move the collection cup out of the way of the liquid jet t

2 Safety6 B-395 Pro Operation Manual, Version C2 SafetyThis chapter points out the safety concept of the instrument and contains general rules of beh

6 Operation60 B-395 Pro Operation Manual, Version C6.11 Heat sterilization of the reaction vessel1. Prepare the Reaction Vessel according to section

6 Operation61 B-395 Pro Operation Manual, Version C6.13 Encapsulation procedure for immobilization of micro-organisms in Ca-alginate beadsIn this sec

6 Operation62 B-395 Pro Operation Manual, Version C8. As soon as the bead chain is stable, move the collection cup out of the way of the liquid jet t

6 Operation63 B-395 Pro Operation Manual, Version C6.14 Encapsulation protocol for alginate-PLL-alginate membranesThe Alginate-Polylysine-Alginate-me

6 Operation64 B-395 Pro Operation Manual, Version CProcedure1. Prepare the reaction vessel and autoclave it as described in section 6.10 and 6.11.2.

6 Operation65 B-395 Pro Operation Manual, Version CSpecial recommendations for cellsFor dividing cells - dissolve the core alginate, then maintain Na/

6 Operation66 B-395 Pro Operation Manual, Version C6.15 Theoretical backgroundEqu. 1: When a laminar jet is mechanically disturbed at the frequency ƒ

6 Operation67 B-395 Pro Operation Manual, Version CFigure 6-5: Influence of the liquid jet velocity and the nozzle diameter on flow rate, as calculated

7 Maintenance and repairs68 B-395 Pro Operation Manual, Version CTable 6-7: Optimal working conditions for the Encapsulator determined with alginate

6 Operation69 B-395 Pro Operation Manual, Version CFigure 6-7: Amount of beads with a diameter of 0.3 to 0.6 mm formed from 1 mL of immobilization mix

2 Safety7 B-395 Pro Operation Manual, Version C2.4 Safety warnings and safety signals used in this manualDANGER, WARNING, CAUTION and NOTICE are stan

6 Operation70 B-395 Pro Operation Manual, Version CFigure 6-9: Amount of cells per bead made from different cell concentrations for bead diameters of

7 Maintenance and repairs71 B-395 Pro Operation Manual, Version C7 Maintenance and repairsThis chapter gives instructions on maintenance work to be

7 Maintenance and repairs72 B-395 Pro Operation Manual, Version C7.4 Cleaning! WarningPressure increasement in the inlet-system due to clogged nozzl

7 Maintenance and repairs73 B-395 Pro Operation Manual, Version CFigure 7-1: Cleaning procedure of the nozzle – Take a syringe containing air on top

8 Troubleshooting74 B-395 Pro Operation Manual, Version C8 Troubleshooting8.1 Malfunctions and their remedyThe table below lists possible operating

9 Shutdown, storage, transport and disposal75 B-395 Pro Operation Manual, Version C9 Shutdown, storage, transport and disposalThis chapter instructs

9 Shutdown, storage, transport and disposal76 B-395 Pro Operation Manual, Version C9.2 DisposalFor instrument disposal in an environmentally friendl

10 Declarations and requirements77 B-395 Pro Operation Manual, Version C10 Declarations and requirements10.1 FCC requirements (for USA and Canada)E

10 Declarations and requirements78 B-395 Pro Operation Manual, Version C10.2 Health and Safety Clearance Health and Safety Clearance Health and Saf

10 Declarations and requirements79 B-395 Pro Operation Manual, Version C10.3 Declaration of conformity

2 Safety8 B-395 Pro Operation Manual, Version CExplosive gases, explosive environmentHarmful to live-formsDevice damagePressurized gas/airWear laborat

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2 Safety9 B-395 Pro Operation Manual, Version C2.5 Product safetySafety warnings in this manual (as described in section 2.4) serve to make the user