Buchi NIRFlex N-500 User Manual download pdf (Page 21)

4 Description of function

21 NIRFlex N-500 Operation Manual, Version F

4.2.1 Transﬂectance mode

Translucent and opaque liquids can be analyzed via transﬂectance mode. The light penetrates the

liquid, is diffusely reﬂected by the reference plate and passes the sample a second time. The trans-

ﬂected rays contain the spectral information of the sample.

Factors that can inﬂuence the measurement:

Not enough sample material available•

Air bubbles in the measurement path and under the transﬂectance cover•

Sample not homogeneous or representative•

Temperature of sample material•

Sample cup or transﬂectance cover inadequate (e.g. cup material and thickness, blind spots, dirt •

etc.)

NIRFlex Solids:

Diffuse reﬂecting / reference plate

Small, deﬁned gap for liquid samples

Detector

NIR light

Sample cup / petri dish

NIRFlex Fiber Optic Solids / SMA:

Diffuse reﬂecting / reference plate

Small gap for liquid samples

Fiber optics (example)

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Buchi NIRFlex N-500 User Manual Page 21