Buchi PrepChrom C-700 User Manual download pdf (Page 78)

6|Operation BÜCHILabortechnikAG

78/110 PrepChromC700

• Stepgradientmode

Note

Thefirststepisusedasastartpointonlyandcorrespondstotheconditionused

duringequilibration(ifactivated).Thetimeofthefirststepcannotbemodified.

u Adjusttheflowrate(1).

Note

WhenusingtheBUCHIcolumnmethods,thesystemsetstheoptimalflowrate

forthecolumn(onlyflashcolumns).

u Adjustthepercentageoftheusedsolvents(2).

Note

Alwaysstartadjustingthesolventpercentagesfromtherighttotheleft.

u Addthesecondstep(3).

u Settheendtimeforthesecondstep(4).

Note

Theflowrateandthepercentageoftheusedsolventsinthesecondstepare

equaltothefirststep(=equilibrationstep)andcannotbemodifiedseparately.

u Addotherstepsforelution(3)andadjusttheflowrateandpercentageof

thesolvents.

u SelectthetabDetection.

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Buchi PrepChrom C-700 User Manual Page 78