BÜCHILabortechnikAG Operation|6
PrepChromC700 81/110
6.5 Equilibrationandinjectionmodes
u Chooseanequilibrationmodetoconditionthecolumnbeforeinjection.
Mode Action
None Nocolumnconditioningbeforeinjec
tion
Equilibration Columnconditioningbeforeinjection;
timedesignation
Equil.+Dryload Columnconditioningbeforeinjection;
notiming;nopossibilityforloopinjec
tionatthe6wayelectricalvalve
u Chooseinjectionmode
Mode Action
Auto Noinjectionthroughtheloopatthe6
wayelectricalvalve;directloadingof
thesampleontopofthecolumn;starts
directlyafterequilibration(ifactivated)
Valveinjection Injectionviatheloopatthe6wayelec
tricalvalve;startsdirectlyafterequili
bration(ifactivated)
Waitforinjection Noinjectionviatheloopatthe6way
electricalvalve;directloadingofthe
sampleontopofthecolumnwhichhas
tobeconfirmedmanually;startsafter
equilibration
Valveinj.+Wait Injectionviatheloopatthe6wayelec
tricalvalvewhichhastobeconfirmed
bytheuser
6.6 Makealiquidinjection
Theloadingandinjectioncanbedonewithoutstoppingthepump.
u Fillasyringewiththevolumeofsamplechosenandplaceitintheinjection
adapterofthe6wayelectricalvalve.
• Keepthesyringeontheadaptertoavoidaleakofthesample.
u Filltheloopwiththecontentofthesyringe.Ifthequantityinjectedis
greaterthanthecapacityoftheloop,thesurplusflowsinthewastebottle.
• Defaultloopvolume:2mL
u Createanewmethodtogetaccesstothesettingsmenu.
• 6.4 Create a new method [} page75]
u SelectthetabSettings.
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