Buchi Rotavapo R-220 SE User Manual download pdf (Page 47)

5 Putting into operation

47 R-220 SE Operation Manual, Version F

All sealings in contact with the product have a PTFE surface and can be disinfected in an ultrasonic

bath with a corresponding disinfection medium. At reinstallation, mind hygienic handling!

NOTE

Do not bring the seals in contact with lubricants or other harmful substances!

After cleaning, the system must be assembled to run a disinfection cycle, see below.

5.10.2 Example for a disinfection via an evaporation cycle

For evaporation under hygienic conditions it is vital to establish a closed processing. Due to the func-

tional principle all in- and outlets to environmental air or inert gas must be sealed with a bacteria ﬁlter.

Preparational steps for disinfection cycles:

• Connect a tube to every aeration valve inlet and install a bacteria ﬁlter at the end of the tube(s) to

prevent contamination by environmental air ingress.

When working under inert conditions:

• Install a ﬁlter between the inert gas source and the gas inlet of the glassware setup.

NOTE

If the system is equipped with two receiving ﬂasks switch between both ﬂasks during the running

disinfection evaporation!

Empty the evaporating and the receiving ﬂask and rinse the evaporating ﬂask with water.

Fill 1.5 l of isopropanol into the evaporating ﬂask and start the evaporation under the following condi-

tions:

Heating bath set to 60 °C, vacuum set to 250 mbar (corresponds to a boiling point of isopropanol of

50 °C), chiller set to 7 °C.

At ﬁrst, switch off the chiller or cooling water until the vapor has passed the whole instrument. Then

switch on the chiller or cooling water and distil off 0.75 l. Afterwards empty the evaporting and the

receiving ﬂask and dry the instrument at a vacuum of < 20 mbar.

5.11 Veriﬁcation of the disinfection process

BÜCHI Labortechnik AG carried out a test to verify the disinfection process.

The samples resulting from the test procedure were sent to the Labor für Hygiene und Praktische

Mikrobiologie AG, Chleematte 14, CH-5243 Müllingen, a Swiss Testing Service STS 486 according to

ISO/IEC 17025. There, a photometric haze measurement according to ISO 4833:2003 was carried

out to determine the germ concentration.

The following test setup was used:

Test instrument: Rotavapor R-220 SE, system conﬁguration D with Vacuum Pump V-710 and recircu-

lating chiller.

Both aeration valves were closed with an aseptic ﬁlter Sartorius Midisart 2000, 0.45 µm and an inline

ﬁlter PAL Kleenpak 1.2 µm was ﬁxed in the vacuum line between instrument and pump.

The following representative test contaminants were used:

• Escherichia coli ATCC 25922

• Staphylococcus aureus subsp. Aureus ATCC 25923

• Saccharomyces cerevisiae NCYC 79

• Geobacillus stearothermophilus

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Buchi Rotavapo R-220 SE User Manual Page 47